Biochemistry. 2003 Apr 15;42(14):4028-34.

The binding potential between the cholera toxin B-oligomer and its receptor.

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Physics Department, University of Vermont, Cook Building, Burlington, Vermont 05405, USA.

Abstract

Employing atomic force microscopy as an in situ molecular force probe, we have measured the binding strength between cholera toxin B-pentamer (ctB) and its membrane receptor, ganglioside G(M1). By application of the basic principle of the reaction rate theory, key parameters of the ligand-receptor binding potential can be obtained from our data. The potential has a well with a spatial span of about 2.5 A and a depth of at least six times the thermal energy at room temperature. The very short range nature of the binding potential leads to the specificity of the ctB-G(M1) coupling.

PMID:: 12680755; [PubMed - indexed for MEDLINE]

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