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Anticancer Res. 2003 Jan-Feb;23(1A):485-90.

The actinomycin D-induced apoptosis in BCR-ABL-positive K562 cells is associated with cytoplasmic translocation and cleavage of RNA helicase A.

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  • 1Division of Haematopathology, Department of Paediatric Laboratory Medicine, Hospital for Sick Children, University of Toronto, Toronto, Canada. mohamed.abdelhaleem@sickkids.ca

Abstract

K562 cells express the fusion protein BCR-ABL and have been shown to be relatively more resistant to apoptosis induction by chemotherapeutic agents. We show that Actinomycin D (Act D) induces time- and dose-dependent apoptosis in K562 cells. Act D causes early activation of caspase-3 followed by inhibition of the expression of the anti-apoptotic proteins BCR-ABL and Bcl-xl. Act D-induced apoptosis is associated with cytoplasmic translocation and cleavage of the multifunctional nuclear protein RNA helicase A (RHA). RHA has roles in transcription and RNA metabolism and has been shown to be cleaved during Fas mediated apoptosis. These results suggest that Act D causes caspase-3 activation and apoptosis in BCR-ABL positive K562 cells and that RHA cytoplasmic translocation and cleavage occur in chemotherapy-induced apoptosis.

PMID:
12680254
[PubMed - indexed for MEDLINE]
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