Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Opt Lett. 2003 Jan 15;28(2):69-71.

Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging.

Author information

  • 1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Abstract

We show that the position of a fluorescent nanoparticle can be measured in three dimensions with subnanometer precision and 100-ms temporal resolution by use of standard epifluorescence video imaging in off-focus mode. The particle can be tracked without feedback in a volume of at least 40 microm x 60 microm x 3 microm. With the technique presented, the structure-mobility relationship of 216-nm latex particle in a porous polymer network was studied in three dimensions.

PMID:
12656488
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Optical Society of America
    Loading ...
    Write to the Help Desk