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Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):289-93.

Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases.

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  • 1ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia. philiph@nature.berkeley.edu

Abstract

A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.

PMID:
12656186
[PubMed - indexed for MEDLINE]
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