Nucleic Acids Res. 2003 Apr 1;31(7):1869-76.

A non-redundant microarray of genes for two related bacteria.

Porwollik S, Frye J, Florea LD, Blackmer F, McClelland M.

Source

Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.

Abstract

A microarray with sequences from the annotated open reading frames (ORFs) in Salmonella enterica subspecies 1, serovar Typhimurium was supplemented with annotated chromosomal ORFs from serovar Typhi that are divergent from Typhimurium (>10% DNA sequence divergence). This non- redundant array was used to (i) measure changes in gene copy number in DNA from actively growing versus stationary Typhi and (ii) to reveal the transcriptional response of Typhi to peroxide, a stress similar to that experienced when they are phagocytosed by macrophages. In S.enterica subspecies 1, pairs of genomes differ in the presence or absence of approximately 10% of their genes. An array twice the size of that needed to cover all ORFs for one genome could carry close homologs of all the ORFs for 10 genomes. Non-redundant DNA arrays could be constructed for any group of closely related organisms that differ by the presence and absence of a few genes.

PMID:: 12655003; [PubMed - indexed for MEDLINE]
PMCID: PMC152813

Free PMC Article

Images from this publication.See all images (2) Free text

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Peroxides

Grant Support

AI34829/AI/NIAID NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Related citations

Determination of the gene content of Salmonella genomes by microarray analysis. [Methods Mol Biol. 2007]
Determination of the gene content of Salmonella genomes by microarray analysis.
Porwollik S, McClelland M. Methods Mol Biol. 2007; 394:89-103.
Identification of core and variable components of the Salmonella enterica subspecies I genome by microarray. [Infect Immun. 2005]
Identification of core and variable components of the Salmonella enterica subspecies I genome by microarray.
Anjum MF, Marooney C, Fookes M, Baker S, Dougan G, Ivens A, Woodward MJ. Infect Immun. 2005 Dec; 73(12):7894-905.
Detection of hilA gene sequences in serovars of Salmonella enterica subspecies enterica. [Mem Inst Oswaldo Cruz. 2002]
Detection of hilA gene sequences in serovars of Salmonella enterica subspecies enterica.
Cardona-Castro N, Restrepo-Pineda E, Correa-Ochoa M. Mem Inst Oswaldo Cruz. 2002 Dec; 97(8):1153-6. Epub 2003 Jan 20.
Review Population structure, origins and evolution of major Salmonella enterica clones. [Infect Genet Evol. 2009]
Review Population structure, origins and evolution of major Salmonella enterica clones.
Lan R, Reeves PR, Octavia S. Infect Genet Evol. 2009 Sep; 9(5):996-1005. Epub 2009 Apr 23.
Review Monitoring gene expression using DNA arrays. [Methods Mol Med. 2003]
Review Monitoring gene expression using DNA arrays.
Ali TR, Li MS, Langford PR. Methods Mol Med. 2003; 71:119-34.

See reviews... See all...

Cited by 28 PubMed Central articles

Systems analysis of multiple regulator perturbations allows discovery of virulence factors in Salmonella. [BMC Syst Biol. 2011]
Systems analysis of multiple regulator perturbations allows discovery of virulence factors in Salmonella.
Yoon H, Ansong C, McDermott JE, Gritsenko M, Smith RD, Heffron F, Adkins JN. BMC Syst Biol. 2011 Jun 28; 5:100. Epub 2011 Jun 28.
Spontaneous excision of the Salmonella enterica serovar Enteritidis-specific defective prophage-like element phiSE14. [J Bacteriol. 2010]
Spontaneous excision of the Salmonella enterica serovar Enteritidis-specific defective prophage-like element phiSE14.
Santiviago CA, Blondel CJ, Quezada CP, Silva CA, Tobar PM, Porwollik S, McClelland M, Andrews-Polymenis HL, Toro CS, Zaldívar M, et al. J Bacteriol. 2010 Apr; 192(8):2246-54. Epub 2010 Feb 19.
Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database. [Microb Drug Resist. 2010]
Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database.
Frye JG, Lindsey RL, Rondeau G, Porwollik S, Long F, McClelland M, Jackson CR, Englen MD, Meinersmann RJ, Berrang ME, et al. Microb Drug Resist. 2010 Mar; 16(1):9-19.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
GEO DataSets
Gene expression and molecular abundance data reported in the current articles that are also included in the curated Gene Expression Omnibus (GEO) DataSets.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A non-redundant microarray of genes for two related bacteria.
A non-redundant microarray of genes for two related bacteria.
Nucleic Acids Res. 2003 Apr 1 ;31(7):1869-76.

PubMed
Newborn hearing screening in infants with cleft palates.
Newborn hearing screening in infants with cleft palates.
Otol Neurotol. 2008 Sep ;29(6):812-5.

PubMed
Reversal of RNA polymerase II ubiquitylation by the ubiquitin protease Ubp3.
Reversal of RNA polymerase II ubiquitylation by the ubiquitin protease Ubp3.
Mol Cell. 2008 May 23 ;30(4):498-506.

PubMed
Neutrophil CD64 expression as a diagnostic marker of bacterial infection in febr...
Neutrophil CD64 expression as a diagnostic marker of bacterial infection in febrile children presenting to a hospital emergency department.
Pediatr Emerg Care. 2008 Nov ;24(11):745-8.

PubMed
A randomized, controlled pilot study of acupuncture treatment for menopausal hot...
A randomized, controlled pilot study of acupuncture treatment for menopausal hot flashes.
Menopause. 2008 Nov-Dec ;15(6):1070-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: