J Immunol. 2003 Apr 1;170(7):3572-6.

Activated, but not resting, T cells can be recognized and killed by syngeneic NK cells.

Rabinovich BA, Li J, Shannon J, Hurren R, Chalupny J, Cosman D, Miller RG.

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Department of Medical Biophysics, Ontario Cancer Institute, Toronto, Canada.

Abstract

We demonstrate that IL-2-activated NK cells or lymphokine-activated killer cells recognize and kill syngeneic CD4(+) and CD8(+) T cells that have been activated by APCs. Induction with APC required TCR-specific Ag, and lysis was perforin mediated. Brefeldin A, which disrupts protein transport, inhibited the sensitivity induced by activation. In BALB/c, expression of NKG2D ligands correlated with lysis and could be inhibited by brefeldin A. As well, addition of anti-NKG2D mAb to a killing assay completely abrogated lysis. Transduction of mouse NKG2D into a human NK cell line, YTSeco, conferred upon it the ability to kill activated BALB/c T cells, indicating that NKG2D is necessary for recognition. Our data provide a basis for studying a role for NK cells in T cell regulation.

PMID:: 12646619; [PubMed - indexed for MEDLINE]

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