Quantitative HPLC analysis of withanolides in Withania somnifera.
Department of Pharmacognosy, University of Innsbruck, Innsbruck 6020, Austria. markus.ganzera@uibk.ac.at
One of the most widely used herbs in Ayurvedic medicine is Ashwaghanda, Withania somnifera, a shrub commonly found on the Indian subcontinent. As this plant is increasingly becoming a popular adaptogenic in the western world, analytical methods for its identification and quality control are in demand. Thus, a HPLC method for the determination of withaferin A and withanolide D was developed. The system was successfully used to investigate the presence of the markers in different W. somnifera plant parts as well as to analyze their content in market products.
PMID: 12628397 [PubMed - indexed for MEDLINE]