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Genome Biol. 2003;4(3):R17. Epub 2003 Feb 18.

MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.

Author information

  • 1Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute, 200 Longwood Avenue, Boston, MA 02115, USA.

Abstract

BACKGROUND:

Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate.

RESULTS:

We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals.

CONCLUSIONS:

MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently.

PMID:
12620102
[PubMed - indexed for MEDLINE]
PMCID:
PMC153457
Free PMC Article

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