J Immunol Methods. 2003 Mar 1;274(1-2):77-82.

Chemiluminescence-based BrdU ELISA to measure DNA synthesis.

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Cardiovascular Research Institute and Department of Medical Physiology, College of Medicine, Texas A&M University System Health Science Center, Medical Research Building, Rm 206A, 702 SW H. K. Dodgen Loop, Temple, TX 76504, USA.

Abstract

We describe a simple, sensitive, nonradioactive, relatively rapid and relatively inexpensive protocol to measure DNA synthesis in cultured cells by a chemiluminescent bromodeoxyuridine (BrdU) enzyme-linked immunosorbent assay (ELISA). We show that it exhibits similar sensitivity and activity as traditional 3H-thymidine incorporation assays and a commercial chemiluminescent BrdU ELISA kit when tested in commonly used cell lines, such as NIH 3T3 cells, mink lung epithelial cells (Mv1LU), and baby hamster kidney (BHK-21) fibroblasts. This assay also exhibits a wider dynamic range than colorimetric BrdU ELISA methods. Besides being a viable, nonradioactive alternative to 3H-thymidine incorporation assays, our BrdU ELISA is less expensive than a commercial chemiluminescent BrdU ELISA kit.

PMID:: 12609534; [PubMed - indexed for MEDLINE]

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Chemical compound information

DNA

MW: 523.36 g/mol

MF: C₁₅H₃₁N₃O₁₃P₂

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