Myeloperoxidase/nitrite-mediated lipid peroxidation of low-density lipoprotein as modulated by flavonoids

FEBS Lett. 2003 Feb 27;537(1-3):146-50. doi: 10.1016/s0014-5793(03)00113-3.

Abstract

In the presence of a H(2)O(2)-generating system, myeloperoxidase (MPO) caused conjugated diene formation in low-density lipoprotein (LDL), indicating lipid peroxidation which was dependent on nitrite but not on chloride. The oxidation of LDL was inhibited by micromolar concentrations of flavonoids such as (-)-epicatechin, quercetin, rutin, taxifolin and luteolin, presumably via scavenging of the MPO-derived NO(2) radical. The flavonoids served as substrates of MPO leading to products with distinct absorbance spectra. The MPO-catalyzed oxidation of flavonoids was accelerated in the presence of nitrite.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Catechin / pharmacology
  • Flavonoids / pharmacology*
  • Humans
  • Hydrogen Peroxide / blood
  • Hydrogen Peroxide / metabolism
  • Kinetics
  • Lipid Peroxidation / physiology*
  • Lipoproteins, LDL / blood*
  • Lipoproteins, LDL / drug effects
  • Lipoproteins, LDL / metabolism*
  • Nitrites / metabolism*
  • Peroxidase / metabolism*
  • Quercetin / pharmacology
  • Rutin / pharmacology

Substances

  • Flavonoids
  • Lipoproteins, LDL
  • Nitrites
  • oxidized low density lipoprotein
  • Rutin
  • Catechin
  • Quercetin
  • Hydrogen Peroxide
  • Peroxidase