Abstract
The small GTPase Rho and its effector ROCK/Rho-kinase regulate actin cytoskeletal reorganization through phosphorylation of the regulatory light chain of myosin II. We previously reported that ROCK co-purified with the actin-binding protein filamin-A from HeLa cells. Here, we show that the pleckstrin homology (PH) domain of ROCK, but not the kinase or coiled-coil domain, interacts with filamin-A. We also determined that the PH domain of ROCK binds to the carboxy-terminal region of filamin-A containing the last 24th repeat. ROCK co-localized with filamin-A at the protrusive cell membranes of HeLa cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Blood Proteins / chemistry
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Blood Proteins / genetics
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Blood Proteins / metabolism
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Cell Membrane / metabolism
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Contractile Proteins / chemistry
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Contractile Proteins / genetics
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Contractile Proteins / metabolism*
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Filamins
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HeLa Cells
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Humans
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Intracellular Signaling Peptides and Proteins
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Microfilament Proteins / chemistry
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism*
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Mutation
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Phosphoproteins / chemistry
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Phosphoproteins / genetics
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Phosphoproteins / metabolism
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Protein Serine-Threonine Kinases / chemistry*
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Repetitive Sequences, Amino Acid
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rho-Associated Kinases
Substances
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Blood Proteins
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Contractile Proteins
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Filamins
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Intracellular Signaling Peptides and Proteins
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Microfilament Proteins
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Phosphoproteins
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Recombinant Proteins
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platelet protein P47
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Protein Serine-Threonine Kinases
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rho-Associated Kinases