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    J Biomed Sci. 2003 Jan-Feb;10(1):98-110.

    Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7.

    Kao HH, Chang MS, Cheng JF, Huang JD.

    Department of Food Nutrition, Chung-Hwa College of Medical Technology, Tainan, Taiwan, ROC.

    The multidrug resistance-associated protein (MRP) subfamily transporters associated with anticancer drug efflux are attributed to the multidrug-resistance of cancer cells. The genomic organization of human multidrug resistance-associated protein 7 (MRP7) was identified. The human MRP7 gene, consisting of 22 exons and 21 introns, greatly differs from other members of the human MRP subfamily. A splicing variant of human MRP7, MRP7A, expressed in most human tissues, was also characterized. The 1.93-kb promoter region of MRP7 was isolated and shown to support luciferase activity at a level 4- to 5-fold greater than that of the SV40 promoter. Basal MRP7 gene expression was regulated by 2 regions in the 5'-flanking region at -1,780-1,287 bp, and at -611 to -208 bp. In Madin-Darby canine kidney (MDCK) cells, MRP7 promoter activity was increased by 226% by genotoxic 2-acetylaminofluorene and 347% by the histone deacetylase inhibitor, trichostatin A. The protein was expressed in the membrane fraction of transfected MDCK cells. Copyright 2003 National Science Council, ROC and S. Karger AG, Basel

    PMID: 12566991 [PubMed - indexed for MEDLINE]

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