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J Microbiol Methods. 2003 Mar;52(3):325-32.

Use of gas chromatography-mass spectrometry/solid phase microextraction for the identification of MVOCs from moldy building materials.

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  • 1Department of Medical Microbiology, Dermatology and Infection, University of Lund, Sölvegatan 23, Sweden.

Abstract

Gas chromatography-mass spectrometry/solid phase microextraction (GC-MS/SPME) was applied to identify microbial volatile organic compounds (MVOCs) in water-damaged, mold-infested building materials (gypsum board papers (n=2), mineral wool, and masonite) and in cultivated molds (Aspergillus penicillioides, Stachybotrys chartarum, and Chaetomium globosum). Three SPME fibers (65-microm PDMS-DVB, 75-microm Carboxen-PDMS, and 70-microm Carbowax-stableflex) designed for automated injection were used of which the latter showed best performance. A number of previously reported MVOCs were detected both in the building materials and the cultivated molds. In addition, methyl benzoate was identified both in the S. chartarum and A. penicillioides cultures and in the building materials. SPME combined with GC-MS may be a useful method for the determination of MVOCs emitted from mold-infested building materials.

PMID:
12531501
[PubMed - indexed for MEDLINE]
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