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J Org Chem. 2003 Jan 24;68(2):609-12.

Click chemistry to construct fluorescent oligonucleotides for DNA sequencing.

Author information

  • 1Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.

Abstract

"Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.

PMID:
12530893
[PubMed - indexed for MEDLINE]
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