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Wei Sheng Yan Jiu. 2001 May;30(3):188-9, back cover.

[Culture and identify the endothelial cells of human umbilical vein].

[Article in Chinese]

Author information

  • 1Department of Nutrition and Food Hygiene, West China University of Medical Sciences, Chengdu 610041, China.

Abstract

In order to improve the routine culture method for vascular endothelial cells, endothelial cells of vein from newborn umbilical cord were successfully isolated with combined using 0.1% collagenase and 0.25% trypsin (1:1). The endothelial cells were further abrated by mechanical method. The composition of culture medium was simplified by not adding vascular endothelial cell growth factor and heparin. The cultured cells were verified as vascular endothelial cells by morphology, ultramicromorphology and immunohistochemistry.

PMID:
12525099
[PubMed - indexed for MEDLINE]
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