Taenia saginata derived synthetic peptides with potential for the diagnosis of bovine cysticercosis

Vet Parasitol. 2003 Jan 20;111(1):83-94. doi: 10.1016/s0304-4017(02)00327-8.

Abstract

Immunity in Taeniids is predominantly antibody mediated and thus many serological immuno-determinants will have potential in both protection and diagnosis. The antigenicity of six peptides derived from four potentially protective molecules cloned from a Taenia saginata oncospheres cDNA library have been evaluated as targets for the specific diagnosis of bovine cysticercosis. The six peptides consist of: two peptides (HP6-2 and HP6-3) derived from the sequence of the 18 kDa surface/secreted oncospheral adhesion antigen identified by McAb-HP6, two peptides (Ts45W-1 and Ts45W-5) derived from the sequence of the T. saginata homologue of the T. ovis 45W protective gene family, one peptide (TS45S-10) derived from a T. saginata sequence with significant similarity to the T. ovis 45S protective antigen, and one peptide (TEG-1) derived from the sequence of the T. saginata homologue of Echinococcus spp. main surface protein. Longitudinal studies indicate that T. saginata infected cattle respond to all six peptides by 3-4 weeks post-infection and that the antibody levels remain high for at least 12 weeks post-infection. As protection against Taeniid parasites is predominantly antibody mediated, some of these six peptides may be of value as immuno-prophylactic tools and hence also in assays to determine resistance to infection with the parasite. For diagnosis, on the other hand, only three peptides (HP6-2, TEG-1 and Ts45S-10) performed with the necessary sensitivity and specificity to determine exposure to infection with T. saginata, and now merit an exhaustive evaluation prior to employment as routine diagnostic tools.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / immunology*
  • Antibody Specificity
  • Antigens, Helminth / immunology*
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / immunology
  • Cattle Diseases / parasitology
  • Cross Reactions
  • Cysticercosis / diagnosis*
  • Cysticercosis / immunology
  • Cysticercosis / veterinary*
  • Female
  • Immune Sera / immunology
  • Male
  • Peptides / chemical synthesis
  • Peptides / immunology*
  • Taenia saginata / immunology*
  • Time Factors

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Immune Sera
  • Peptides