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Plant Mol Biol. 2002 Dec;50(6):855-70.

Analysing protein-protein interactions with the yeast two-hybrid system.

Author information

  • 1School of Biology, University of Leeds, Leeds LS2 9J7, UK. b.e.causier@leeds.ac.uk

Abstract

Plant research is moving into the post-genomic era. Proteomic-based strategies are now being developed to study functional aspects of the genes predicted from the various genome-sequencing initiatives. All biological processes depend on interactions formed between proteins and the mapping of such interactions on a global scale is providing interesting functional insights. One of the techniques that has proved itself invaluable in the mapping of protein-protein interactions is the yeast two-hybrid system. This system is a sensitive molecular genetic approach for studying protein-protein interactions in vivo. In this review we will introduce the yeast two-hybrid system, discuss modifications of the system that may be of interest to the plant science community and suggest potential applications of the technology.

PMID:
12516858
[PubMed - indexed for MEDLINE]
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