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Mass Spectrom Rev. 2002 May-Jun;21(3):163-82.

Analysis of protein-nucleic acid interactions by photochemical cross-linking and mass spectrometry.

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  • 1Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.

Abstract

Photochemical cross-linking is a commonly used method for studying the molecular details of protein-nucleic acid interactions. Photochemical cross-linking aids in defining nucleic acid binding sites of proteins via subsequent identification of cross-linked protein domains and amino acid residues. Mass spectrometry (MS) has emerged as a sensitive and efficient analytical technique for determination of such cross-linking sites in proteins. The present review of the field describes a number of MS-based approaches for the characterization of cross-linked protein-nucleic acid complexes and for sequencing of peptide-nucleic acid heteroconjugates. The combination of photochemical cross-linking and MS provides a fast screening method to gain insights into the overall structure and formation of protein-oligonucleotide complexes. Because the analytical methods are continuously refined and protein structural data are rapidly accumulating in databases, we envision that many protein-nucleic acid assemblies will be initially characterized by combinations of cross-linking methods, MS, and computational molecular modeling.

Copyright 2002 Wiley Periodicals, Inc., Mass Spec Rev 21:163-182, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mas.10024

PMID:
12476441
[PubMed - indexed for MEDLINE]
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