Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nucleic Acids Res. 2002 Dec 1;30(23):e130.

Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support.

Author information

  • 1Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology (formerly Centre for Biochemical Technology), Mall Road, Delhi University Campus, Delhi 110 007, India.

Abstract

Polyamine-assisted deprotection conditions have been developed for the rapid and clean cleavage of oligonucleotide chains from a cis-diol group bearing universal polymer support, making it compatible with modern oligonucleotide synthesis via all types of phosphoramidite synthons, including base labile protecting group bearing synthons as well. The synthesized oligonucleotides were found to be comparable with the corresponding standard oligomers with respect to their retention time on HPLC, mass on MALDI-TOF and biological activity in PCR amplification.

PMID:
12466562
[PubMed - indexed for MEDLINE]
PMCID:
PMC137985
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk