DNA Res. 2002 Oct 31;9(5):179-88.

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.

Okazaki N, Kikuno R, Ohara R, Inamoto S, Hara Y, Nagase T, Ohara O, Koga H.

Source

Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.

Abstract

We have been conducting a human cDNA project to predict protein-coding sequences in long cDNAs (> 4 kb) since 1994. The number of these newly identified human genes exceeds 2000 and these genes are known as KIAA genes. As an extension of this project, we herein report characterization of cDNAs derived from mouse KIAA-homologous genes. A primary aim of this study was to prepare a set of mouse. KIAA-homologous cDNAs that could be used to analyze the physiological roles of KIAA genes in mice. In addition, comparison of the structures of mouse and human KIAA cDNAs might enable us to evaluate the integrity of KIAA cDNAs more convincingly. In this study, we selected mouse KIAA-homologous cDNA clones to be sequenced by screening a library of terminal sequences of mouse cDNAs in size-fractionated libraries. We present the entire sequences of 100 cDNA clones thus selected and predict their protein-coding sequences. The average size of the 100 cDNA sequences reached 5.1 kb and that of mouse KIAA-homologous proteins predicted from these cDNAs was 989 amino acid residues.

PMID:: 12465718; [PubMed - indexed for MEDLINE]

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DNA, Complementary

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Related citations in PubMed

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. [DNA Res. 2003]
Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S, Nakajima D, Nagase T, Ohara O, Koga H. DNA Res. 2003 Feb 28; 10(1):35-48.
Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. [DNA Res. 2003]
Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H. DNA Res. 2003 Aug 31; 10(4):167-80.
Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. [DNA Res. 2004]
Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, et al. DNA Res. 2004 Jun 30; 11(3):205-18.
Review Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. [DNA Res. 2002]
Review Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.
Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T. DNA Res. 2002 Jun 30; 9(3):99-106.
Review Kazusa mammalian cDNA resources: towards functional characterization of KIAA gene products. [Brief Funct Genomic Proteomic....]
Review Kazusa mammalian cDNA resources: towards functional characterization of KIAA gene products.
Nagase T, Koga H, Ohara O. Brief Funct Genomic Proteomic. 2006 Mar; 5(1):4-7. Epub 2006 Feb 20.

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Cited by 1 PubMed Central article

HUGE: a database for human KIAA proteins, a 2004 update integrating HUGEppi and ROUGE. [Nucleic Acids Res. 2004]
HUGE: a database for human KIAA proteins, a 2004 update integrating HUGEppi and ROUGE.
Kikuno R, Nagase T, Nakayama M, Koga H, Okazaki N, Nakajima D, Ohara O. Nucleic Acids Res. 2004 Jan 1; 32(Database issue):D502-4.

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Prediction of the coding sequences of mouse homologues of KIAA gene: I. The comp...
Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA Res. 2002 Oct 31 ;9(5):179-88.

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