Nat Rev Mol Cell Biol. 2002 Dec;3(12):964-70.

Protein engineering 20 years on.

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Department of Chemistry, University of York, York YO10 5YW, UK. jab@ysbl.york.ac.uk

Abstract

It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.

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