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Pyrosequencing for microbial typing.

Author information

  • 1Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto 94304, USA. mostafa@stanford.edu

Abstract

Pyrosequencing is a real-time DNA sequencing technique generating short reads rapidly and inexpensively. This technology has the potential advantage of accuracy, ease-of-use, high flexibility and is now emerging as a popular platform for microbial typing. Here, we review the methodology and the use of this technique for viral typing, bacterial typing, and fungal typing. In addition, we describe how to use multiplexing for accurate and rapid typing.

PMID:
12457996
[PubMed - indexed for MEDLINE]
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