Nat Genet. 2002 Dec;32 Suppl:509-14.

Post-analysis follow-up and validation of microarray experiments.

Chuaqui RF, Bonner RF, Best CJ, Gillespie JW, Flaig MJ, Hewitt SM, Phillips JL, Krizman DB, Tangrea MA, Ahram M, Linehan WM, Knezevic V, Emmert-Buck MR.

Source

Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Abstract

Measurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.

PMID:: 12454646; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Medical

ClinicalTrials.gov

Supplemental Content

Save items

Related citations in PubMed

Profound normalisation challenges remain in the analysis of data from microarray experiments. [Appl Bioinformatics. 2003]
Profound normalisation challenges remain in the analysis of data from microarray experiments.
Lyons-Weiler J. Appl Bioinformatics. 2003; 2(4):193-5.
An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression. [Nucleic Acids Res. 2001]
An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression.
Yue H, Eastman PS, Wang BB, Minor J, Doctolero MH, Nuttall RL, Stack R, Becker JW, Montgomery JR, Vainer M, et al. Nucleic Acids Res. 2001 Apr 15; 29(8):E41-1.
The value of microarray techniques for quantitative gene profiling in molecular diagnostics. [Trends Mol Med. 2002]
The value of microarray techniques for quantitative gene profiling in molecular diagnostics.
Bustin SA, Dorudi S. Trends Mol Med. 2002 Jun; 8(6):269-72.
Review Strategies for microarray analysis of limiting amounts of RNA. [Brief Funct Genomic Proteomic....]
Review Strategies for microarray analysis of limiting amounts of RNA.
Livesey FJ. Brief Funct Genomic Proteomic. 2003 Apr; 2(1):31-6.
Review Standards in gene expression microarray experiments. [Methods Enzymol. 2006]
Review Standards in gene expression microarray experiments.
Salit M. Methods Enzymol. 2006; 411:63-78.

See reviews... See all...

Cited by 53 PubMed Central articles

Aberrant phenotypes of transgenic mice expressing dimeric human erythropoietin. [Reprod Biol Endocrinol. 2012]
Aberrant phenotypes of transgenic mice expressing dimeric human erythropoietin.
Yun SJ, Naidansuren P, Sim BW, Park JJ, Park CW, Nanjidsuren T, Kang MH, Hwang SY, Yoon JT, Min KS. Reprod Biol Endocrinol. 2012 Jan 27; 10:6. Epub 2012 Jan 27.
GiSAO.db: a database for ageing research. [BMC Genomics. 2011]
GiSAO.db: a database for ageing research.
Hofer E, Laschober GT, Hackl M, Thallinger GG, Lepperdinger G, Grillari J, Jansen-Dürr P, Trajanoski Z. BMC Genomics. 2011 May 24; 12:262. Epub 2011 May 24.
Ecological genetics and genomics of plant defenses: Evidence and approaches. [Funct Ecol. 2011]
Ecological genetics and genomics of plant defenses: Evidence and approaches.
Anderson JT, Mitchell-Olds T. Funct Ecol. 2011 Apr; 25(2):312-324.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Post-analysis follow-up and validation of microarray experiments.
Post-analysis follow-up and validation of microarray experiments.
Nat Genet. 2002 Dec ;32 Suppl:509-14.

PubMed
Microarray databases: standards and ontologies.
Microarray databases: standards and ontologies.
Nat Genet. 2002 Dec ;32 Suppl:469-73.

PubMed
Parallel cloning, expression, purification and crystallization of human proteins...
Parallel cloning, expression, purification and crystallization of human proteins for structural genomics.
Acta Crystallogr D Biol Crystallogr. 2002 Dec ;58(Pt 12):2102-8. Epub 2002 Nov 23 .

PubMed
Treatment for anthracycline-pretreated metastatic breast cancer.
Treatment for anthracycline-pretreated metastatic breast cancer.
Oncologist. 2002 ;7 Suppl 6:4-12.

PubMed
Comparison of two commercial assays with expert microscopy for confirmation of s...
Comparison of two commercial assays with expert microscopy for confirmation of symptomatically diagnosed malaria.
J Clin Microbiol. 2002 Dec ;40(12):4675-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: