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1: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jan 5;783(1):133-40.Click here to read Links

High-performance liquid chromatography coupled with negative ion tandem mass spectrometry for determination of pravastatin in human plasma.

Zhu Z, Neirinck L.

Pharmascience, 6111 Royalmount Ave., Suite 100, Quebec, H4P 2T4, Montreal, Canada. zzhu@pharmascience.com

A new method, using high-performance liquid chromatography/ion electrospray (negative ion) mass spectrometry, has been developed for the determination of a hydrophilic liver-specific inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase, pravastatin in human plasma. In this method, plasma samples were prepared by a solid-phase extraction on C(18) Bond Elut cartridge. Chromatography was carried out with a Zorbax C(8) column. Simple isocratic chromatography conditions were used. The method has been validated in a linear range of 0.25-300 ng/ml with a coefficient of variation of 0.6-3.4%. The overall recovery was 90.5% for pravastatin and 90.8% for the internal standard beta-hydroxy-lovastatin. The method is simple and reliable with a total run time of less than 2 min.

PMID: 12450532 [PubMed - indexed for MEDLINE]

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