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1: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jan 5;783(1):133-40.Click here to read Links

High-performance liquid chromatography coupled with negative ion tandem mass spectrometry for determination of pravastatin in human plasma.

Pharmascience, 6111 Royalmount Ave., Suite 100, Quebec, H4P 2T4, Montreal, Canada. zzhu@pharmascience.com

A new method, using high-performance liquid chromatography/ion electrospray (negative ion) mass spectrometry, has been developed for the determination of a hydrophilic liver-specific inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase, pravastatin in human plasma. In this method, plasma samples were prepared by a solid-phase extraction on C(18) Bond Elut cartridge. Chromatography was carried out with a Zorbax C(8) column. Simple isocratic chromatography conditions were used. The method has been validated in a linear range of 0.25-300 ng/ml with a coefficient of variation of 0.6-3.4%. The overall recovery was 90.5% for pravastatin and 90.8% for the internal standard beta-hydroxy-lovastatin. The method is simple and reliable with a total run time of less than 2 min.

PMID: 12450532 [PubMed - indexed for MEDLINE]

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