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Biochem Soc Trans. 2002 Nov;30(Pt 6):1056-9.

Investigations into the regulation of lipid biosynthesis in Brassica napus using antisense down-regulation.

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  • 1School of Biological and Biomedical Sciences, University of Durham, South Road, UK.


We have generated antisense plants of Brassica napus, targeting either the cytoplasmic Type I acetyl-CoA carboxylase or the beta-ketoacyl-acyl carrier protein reductase of the Type II dissociable fatty acid synthase, in order to investigate the importance of these components in regulating lipid metabolism in plants. Using a range of down-regulated plants, it has become clear that down-regulation of these genes also causes down-regulation of other components of lipid metabolism, at the activity, translational and transcriptional levels. These plants exhibit effects, not immediately predicted, on oil yield and carbon resource allocation in seeds.

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