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Biochem Soc Trans. 2002 Nov;30(Pt 6):1056-9.

Investigations into the regulation of lipid biosynthesis in Brassica napus using antisense down-regulation.

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  • 1School of Biological and Biomedical Sciences, University of Durham, South Road, UK. A.R.Slabas@durham.ac.uk

Abstract

We have generated antisense plants of Brassica napus, targeting either the cytoplasmic Type I acetyl-CoA carboxylase or the beta-ketoacyl-acyl carrier protein reductase of the Type II dissociable fatty acid synthase, in order to investigate the importance of these components in regulating lipid metabolism in plants. Using a range of down-regulated plants, it has become clear that down-regulation of these genes also causes down-regulation of other components of lipid metabolism, at the activity, translational and transcriptional levels. These plants exhibit effects, not immediately predicted, on oil yield and carbon resource allocation in seeds.

PMID:
12440971
DOI:
10.1042/
[PubMed - indexed for MEDLINE]

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