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Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15393-7. Epub 2002 Nov 18.

Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold.

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  • 1Department of Physics, University of Illinois, Chicago 60607-7059, USA.


Isolated newt (Notophthalmus viridescens) chromosomes were studied by using micromechanical force measurement during nuclease digestion. Micrococcal nuclease and short-recognition-sequence blunt-cutting restriction enzymes first remove the native elastic response of, and then to go on to completely disintegrate, single metaphase newt chromosomes. These experiments rule out the possibility that the mitotic chromosome is based on a mechanically contiguous internal non-DNA (e.g., protein) "scaffold"; instead, the mechanical integrity of the metaphase chromosome is due to chromatin itself. Blunt-cutting restriction enzymes with longer recognition sequences only partially disassemble mitotic chromosomes and indicate that chromatin in metaphase chromosomes is constrained by isolated chromatin-crosslinking elements spaced by approximately 15 kb.

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