Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15357-62. Epub 2002 Nov 15.

Structure and function from the circadian clock protein KaiA of Synechococcus elongatus: a potential clock input mechanism.

Author information

  • 1Department of Biology, Texas A&M University, College Station 77843, USA.

Erratum in

  • Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):763..

Abstract

In the cyanobacterium Synechococcus elongatus (PCC 7942) the proteins KaiA, KaiB, and KaiC are required for circadian clock function. We deduced a circadian clock function for KaiA from a combination of biochemical and structural data. Both KaiA and its isolated carboxyl-terminal domain (KaiA180C) stimulated KaiC autophosphorylation and facilitated attenuation of KaiC autophosphorylation by KaiB. An amino-terminal domain (KaiA135N) had no function in the autophosphorylation assay. NMR structure determination showed that KaiA135N is a pseudo-receiver domain. We propose that this pseudo-receiver is a timing input-device that regulates KaiA stimulation of KaiC autophosphorylation, which in turn is essential for circadian timekeeping.

PMID:
12438647
[PubMed - indexed for MEDLINE]
PMCID:
PMC137721
Free PMC Article

Images from this publication.See all images (5)Free text

Fig 1.
Fig 2.
Fig 3.
Fig 4.
Fig 5.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk