Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Spine (Phila Pa 1976). 2002 Nov 15;27(22):2484-90.

Immunolocalization of phagocytic cells in normal and degenerated intervertebral discs.

Author information

  • 1Institute of Pathology, Academic Hospital Munich-Bogenhausen, M√ľnchen, Germany. Andreas.Nerlich@lrz. uni-muenchen.de

Abstract

STUDY DESIGN:

An immunohistochemical study on human autopsy lumbar intervertebral discs and surgical specimens was conducted.

OBJECTIVE:

To investigate the presence of phagocytic cells within herniated and nonherniated disc tissue and its correlation with disc calcification and degeneration.

SUMMARY OF BACKGROUND DATA:

Increasing knowledge is gathered on the molecular mechanisms of extracellular matrix degradation during the process of disc degeneration. However, the data available on the cells involved in this process are sparse.

METHODS:

Three different study populations were investigated: 1) 31 midsagittal tissue slices (age range, 0-86 years) encompassing the complete motion segment were decalcified and stained with a monoclonal antibody against the lysosomal CD68 antigen; 2) 12 additional midsagittal (undecalcified) tissue slices from normal fresh cadavers were resin-embedded and used for colocalization of calcifications (Kossa staining) and CD68-positive cells; and 3) in 53 surgical disc specimens from 32 individuals undergoing lumbar surgical interventions, the abundance of CD68-positive cells was correlated with diagnostic groups and magnetic resonance image findings.

RESULTS:

In the discs of fetuses, infants, and adolescents, no labeled cells were seen. However, CD68-positive cells were detected in the nucleus pulposus of all individuals with histomorphologic signs of disc degeneration, predominantly in discs adjacent to cleft formations. Morphologically, CD68-positive cells did not differ from nuclear chondrocytes. In the anulus fibrosus, CD68-positive cells were seen less frequently. In the resin-embedded specimens, CD68-positive cells were not associated with tissue calcifications. In most of the surgical specimens, positive cells in cluster-like arrangements were seen frequently, particularly in areas of vascular ingrowth.

CONCLUSIONS:

This is the first study describing the abundant presence of CD68-positive cells in human nonherniated disc nucleus pulposus. The findings additionally suggests that these cells are not invaded monocytes or macrophages, but rather, transformed resident cells. It is assumed that these cells are involved in the phagocytosis of extracellular matrix, and that discal cells therefore promote disc degradation, ultimately leading to a loss of biomechanical properties.

Comment in

PMID:
12435979
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Lippincott Williams & Wilkins
    Loading ...
    Write to the Help Desk