Display Settings:

Format

Send to:

Choose Destination
    Science. 2002 Nov 8;298(5596):1209-13.

    Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography.

    Source

    Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany.

    Abstract

    Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.

    PMID:
    12424373
    [PubMed - indexed for MEDLINE]

      Supplemental Content

      Save items

      loading

      Recent activity

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      Write to the Help Desk