Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nature. 2002 Oct 31;419(6910):944-7.

Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota.

Author information

  • 1INSERM U373, Faculté de Médecine Necker-Enfants Malades, 156 rue de Vaugirard, 75730, Paris Cedex 15, France.

Abstract

Somatic hypermutation of immunoglobulin genes is a unique, targeted, adaptive process. While B cells are engaged in germinal centres in T-dependent responses, single base substitutions are introduced in the expressed Vh/Vl genes to allow the selection of mutants with a higher affinity for the immunizing antigen. Almost every possible DNA transaction has been proposed to explain this process, but each of these models includes an error-prone DNA synthesis step that introduces the mutations. The Y family of DNA polymerases--pol eta, pol iota, pol kappa and rev1--are specialized for copying DNA lesions and have high rates of error when copying a normal DNA template. By performing gene inactivation in a Burkitt's lymphoma cell line inducible for hypermutation, we show here that somatic hypermutation is dependent on DNA polymerase iota.

PMID:
12410315
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk