Abstract
In higher plants, long-chain fatty acid hydroperoxides are intermediates in the synthesis of a diverse group of bioactive compounds. We used the reverse trascriptase-polymerase chain reaction to isolate a gene responsible for the oxidization of fatty acids from Petunia hybrida. A P450 cDNA not isolated earlier, CYP92B1, contained an open reading frame predicted to encode a polypeptide consisting of 510 amino acid residues. The transcript of the cyp92B1 gene was expressed at a high level in the early stage of flower development. CYP92B1 cDNA was expressed in a yeast, Saccharomyces cerevisiae, and recombinant yeast microsomes containing CYP92B1, a hemoprotein, metabolized lauric acid, linoleic acid, and linolenic acid.
MeSH terms
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5' Flanking Region / genetics
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Amino Acid Sequence
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Base Sequence
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Blotting, Southern
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Catalysis
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Chromatography, Thin Layer
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Cloning, Molecular
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Cytochrome P-450 Enzyme System / chemistry
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Cytochrome P-450 Enzyme System / genetics*
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Cytochrome P-450 Enzyme System / metabolism*
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DNA, Complementary / genetics
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Fatty Acids / chemistry*
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Fatty Acids / metabolism*
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Flowers / enzymology*
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Flowers / genetics
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Flowers / growth & development
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Gene Expression Regulation, Plant
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Lauric Acids / metabolism
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Linoleic Acid / metabolism
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Molecular Sequence Data
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Oxidation-Reduction
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Petunia / enzymology*
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Petunia / genetics
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Petunia / growth & development
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Yeasts / genetics
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Yeasts / metabolism
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alpha-Linolenic Acid / metabolism
Substances
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DNA, Complementary
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Fatty Acids
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Lauric Acids
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alpha-Linolenic Acid
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lauric acid
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Cytochrome P-450 Enzyme System
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Linoleic Acid