CYP92B1, A cytochrome P450, expressed in petunia flower buds, that catalyzes monooxidation of long-chain fatty acids

Biosci Biotechnol Biochem. 2002 Sep;66(9):1819-28. doi: 10.1271/bbb.66.1819.

Abstract

In higher plants, long-chain fatty acid hydroperoxides are intermediates in the synthesis of a diverse group of bioactive compounds. We used the reverse trascriptase-polymerase chain reaction to isolate a gene responsible for the oxidization of fatty acids from Petunia hybrida. A P450 cDNA not isolated earlier, CYP92B1, contained an open reading frame predicted to encode a polypeptide consisting of 510 amino acid residues. The transcript of the cyp92B1 gene was expressed at a high level in the early stage of flower development. CYP92B1 cDNA was expressed in a yeast, Saccharomyces cerevisiae, and recombinant yeast microsomes containing CYP92B1, a hemoprotein, metabolized lauric acid, linoleic acid, and linolenic acid.

MeSH terms

  • 5' Flanking Region / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Catalysis
  • Chromatography, Thin Layer
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / chemistry
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism*
  • DNA, Complementary / genetics
  • Fatty Acids / chemistry*
  • Fatty Acids / metabolism*
  • Flowers / enzymology*
  • Flowers / genetics
  • Flowers / growth & development
  • Gene Expression Regulation, Plant
  • Lauric Acids / metabolism
  • Linoleic Acid / metabolism
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Petunia / enzymology*
  • Petunia / genetics
  • Petunia / growth & development
  • Yeasts / genetics
  • Yeasts / metabolism
  • alpha-Linolenic Acid / metabolism

Substances

  • DNA, Complementary
  • Fatty Acids
  • Lauric Acids
  • alpha-Linolenic Acid
  • lauric acid
  • Cytochrome P-450 Enzyme System
  • Linoleic Acid