Abstract
Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor.
MeSH terms
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Animals
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Cells, Cultured
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Chondrocytes / physiology*
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Cyclooxygenase 2
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DNA, Complementary
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Dose-Response Relationship, Drug
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Gene Expression Regulation*
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Gene Library
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In Situ Hybridization / methods
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Isoenzymes / genetics
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Matrix Metalloproteinase 3 / genetics
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Myeloid Cell Leukemia Sequence 1 Protein
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Neoplasm Proteins / drug effects
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Neoplasm Proteins / genetics
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Nitric Oxide / metabolism*
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Nitric Oxide / pharmacology
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Nitric Oxide Donors / pharmacology
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Prostaglandin-Endoperoxide Synthases / genetics
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Proto-Oncogene Proteins c-bcl-2*
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Rabbits
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Rats
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Rats, Wistar
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S-Nitrosoglutathione / pharmacology
Substances
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DNA, Complementary
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Isoenzymes
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Mcl1 protein, rat
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Myeloid Cell Leukemia Sequence 1 Protein
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Neoplasm Proteins
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Nitric Oxide Donors
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Proto-Oncogene Proteins c-bcl-2
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Nitric Oxide
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S-Nitrosoglutathione
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Cyclooxygenase 2
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Prostaglandin-Endoperoxide Synthases
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Matrix Metalloproteinase 3