Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Immunol. 2002 Nov;3(11):1090-6. Epub 2002 Oct 21.

Imaging antigen-induced PI3K activation in T cells.

Author information

  • 1Département de Biologie Cellulaire, Institut Cochin, INSERM U567, CNRS UMR 7627, Université René Descartes, 22 rue Méchain, 75014 Paris, France.

Abstract

Activation of phosphoinositide 3-kinase (PI3K) at the immunological synapse between a T cell and an antigen-presenting cell (APC) has not been demonstrated. Using fluorescent-specific probes, we show here that the formation of an immunological synapse led to sustained production of 3'-phosphoinositides in the T cell, whereby phosphatidylinositol-3,4,5-trisphosphate (PIP3) but not phosphatidylinositol-3,4-bisphosphate was localized to the cell membrane. The accumulation of PIP3 after T cell activation preceded the increase in intracellular calcium. Neither the formation of conjugates between T cells and APCs nor signaling events such as phosphotyrosine accumulation and calcium increase changed substantially when PI3K was inhibited, and only a limited reduction in synthesis of interleukin 2 occurred. In T cell-APC conjugates, PIP3 accumulated at the T cell-APC synapse as well as in the rest of the T cell plasma membrane, which indicated unusual regulation of PI3K activity during antigen presentation.

Comment in

  • Slick signaling. [Nat Immunol. 2002]
PMID:
12389041
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk