The alignment includes (1) all known members of human SUV39 family: SUV39H1 (NP_003164), SUV39H2 (NP_078946), G9a (S30385), Eu-HMT1 (AAM09024), SETDB1 (NP_036564), and CLLL8 (NP_114121); (2) proteins (in bold) that have been shown to have HKMT activity from various species: N. crassa (Nc) DIM-5 (AAL35215), S. pombe (Sp) Clr4 (060016), A. thaliana (At) SUVH4 or KRYPTONITE (AAK28969), S. cerevisiae (Sc) SET1 (P38827) and SET2 (P46995), human SET7 (XP_040150) and PR-SET7 (AAL40879); (3) three bacterial SET proteins: Xylella fastidiosa (Xf) SET (AAF84287), Bradyrhizobium japonicum (Bj) SET (Q9ANB6), and Chlamydophila pneumoniae (Cp) SET (AAD19016); and (4) human EZH2 protein, which appears inactive in vitro (Rea et al., 2000). The residue number and secondary structural elements of DIM-5 (helices A–J and strands 1–17) are shown above the aligned sequences. Dashed lines indicate disordered regions. The color coding is light blue for the N terminus (residues 25–62), yellow for the pre-SET (residues 63–146), green for the SET (residues 147–236 and 248–277), magenta for the signature motifs (SET residues 237–247 and 278–285), and gray for the post-SET (residues 299–308). The amino acids highlighted are invariant (white against black) and conserved (white against gray) among almost all members of the SUV39 family. The number in parentheses indicates the number of amino acids inserted relative to the alignment. The lowercase letters above the sequences indicate the structural/functional role of the corresponding DIM-5 residues: “h” indicates intramolecular hydrophobic interaction, “n” indicates intramolecular nonhydrophobic (polar or charge) interaction, “z” indicates zinc coordination, asterisk indicates structural residue Gly or Pro, and “s” indicates surface-exposed residues potentially important for cofactor or substrate binding or catalysis. The red circles mark the residues that were mutated in this study.

(A) Front view of ribbons diagram (Carson, 1997) (top, stereo; bottom, mono). The protein is colored according to Figure 1, and the three zinc ions are red, green, and blue balls (as in C). The difference electron density map (black), contoured at 5.5σ above the mean, indicates the presumed cofactor binding site (supported by tests on mutant forms).
(B) Side view. A dashed line indicates the disordered amino acids between strand β17 (magenta) and the post-SET segment (gray).
(C) Stereo diagram of the triangular zinc cluster. Three zinc ions are colored in red, green, and blue, the bridging cysteine residues are colored in orange, and the nonbridging cysteine residues are colored to match their associated zinc ion. The atoms are gray (carbon) and yellow (sulfur). The pre-SET sequence of DIM-5 is shown above. Both Cys-rich segments coordinate the red and blue zinc ions jointly, while the green zinc ion is coordinated solely by the five-Cys segment.

HKMT activity as functions of (A) temperature, (B) salt concentration, (C) pH, and (D) AdoMet crosslinking as a function of pH. The buffers used were 50 mM Na citrate for pH 5.0–6.0, MES for pH 6.0–6.5, HEPES for pH 7.0–7.5, Tris for pH 8.0–8.5, Bicine for pH 9.0, and glycine for pH 9.35–10.7. To rule out the potential inhibitory effect of Na citrate, both Na citrate and Mes are used for pH 6.0.
(E) Activities of DIM-5 mutants with conservative point mutations. All mutant proteins were expressed to level similar to that of the wild-type, though some were less soluble, and all were monomeric, suggesting that none of the mutations caused gross aggregation of the protein. Various amounts of mutant enzymes were used, the activities were compared to that of serial dilutions of wild-type enzymes purified in the same way, and the specific activity of mutant proteins relative to wild-type was estimated. The activities shown are averages of at least two measurements.
(F) Fluorographic results of an AdoMet crosslinking experiment at pH 8.0 are shown along with results of Coomassie staining to control for the amount of mutant protein tested.

Close-up view of the proposed cofactor binding site and the adjacent active site (top, stereo; bottom, mono). The difference electron density map (blue) is contoured at 5.5σ; the water molecules are numbered 1–4. Dashed lines indicate the hydrogen bonds. The residues are colored as in Figure 1. The water at site 2 is hydrogen bonded to the main chain carbonyl oxygen atom of R238 and to the water molecules at sites 1 and 3, which in turn interacts with the side chain carbonyl oxygen of N241 and the side chain hydroxyl oxygen of Y204, respectively.

(A) Front view of GRASP surface (Nicholls et al., 1991). The difference electron density map (black) is contoured at 5.5σ. Strand β10 is green, N241, H242, and Y283 are magenta, and C244 is yellow.
(B) Superimposition of Drosophila HP1 β strand (light gray) (Jacobs and Khorasanizadeh, 2002; PDBcode 1KNA) and DIM-5 strand β10 (green). Dashed lines indicate the hydrogen bonds between HP1 and H3 peptide (yellow). The DIM-5 residues on the other side of the HP1 peptide are colored magenta. The dimethylated (methyl groups in black) target nitrogen atom occupies water site 2 (see Figure 4). The sequence of histone H3 peptide is shown at the bottom; both K4 and K14 are five residues away from K9.
(C) The docked H3 peptide lies in the putative peptide binding cleft. The cleft extends in both directions following turns as indicated. Surface charge distribution is displayed as blue for positive, red for negative, and white for neutral.
(D) Superimposition of active site NPPY residues of TaqI DNA-adenine amino MTase (gray) (Goedecke et al., 2001; PDB code 1G38) and the proposed DIM-5 active site residues N241, H242, and Y283 (magenta). The Tyr in both cases is hydrogen bonded to a main chain amide nitrogen atom (dashed bonds).

(A) Analysis of zinc content of DIM-5 with and without EDTA treatment. DIM-5 protein was incubated with 20 mM EDTA for 2 days, at which time HKMT activity was no longer detectable. To remove zinc bound to EDTA, the protein was either dialyzed (Exp1) or subjected to gel filtration chromatography (Exp2) against 20 mM glycine (pH 9.8), 5% glycerol, 0.5 mM DTT, and 1 mM EDTA.
(B) Purified DIM-5 protein (1 mg/ml in 20 mM glycine [pH 9.8], 5% glycerol) was incubated with various concentration of 1,10-phenanthroline or EDTA for 18 hr at 4°C. The enzyme was diluted 80-fold and assayed for HKMT activity under standard conditions, except that no DTT was present.
(C) Fluorographic results of AdoMet crosslinking in the presence of EDTA.