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Science. 2002 Oct 4;298(5591):216-8.

Excess polymorphisms in genes for membrane proteins in Plasmodium falciparum.

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  • 1Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA.

Abstract

The detection of single-nucleotide polymorphisms in pathogenic microorganisms has normally been carried out by trial and error. Here we show that DNA hybridization with high-density oligonucleotide arrays provides rapid and convenient detection of single-nucleotide polymorphisms in Plasmodium falciparum, despite its exceptionally high adenine-thymine (AT) content (82%). A disproportionate number of polymorphisms are found in genes encoding proteins associated with the cell membrane. These genes are targets for only 22% of the oligonucleotide probes but account for 69% of the polymorphisms. Genetic variation is also enriched in subtelomeric regions, which account for 22% of the chromosome but 76% of the polymorphisms.

PMID:
12364807
[PubMed - indexed for MEDLINE]
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