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    FEMS Microbiol Lett. 2002 Sep 10;214(2):205-9.

    Cellulomonas flavigena: characterization of an endo-1,4-xylanase tightly induced by sugarcane bagasse.

    Source

    Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 14-740, Mexico D.F. 07300, Mexico.

    Abstract

    Xylanases, an important group of enzymes for biomass degradation in the industry, are commonly found forming complex multienzyme systems. As a preliminary step to the construction of efficient xylanase producers using genetic engineering, we have characterized a gene encoding an endo-beta-1,4 xylanase (xyncflA) from Cellulomonas flavigena. The xylanase activity and the xyncflA synthesis were higher when C. flavigena was grown on sugarcane bagasse. In this substrate, both activity and transcript increased with approximately the same rate during the culture period. When C. flavigena grew on glucose, low signal of mRNA was observed, suggesting that the xyncflA gene is regulated at the transcriptional level.

    PMID:
    12351232
    [PubMed - indexed for MEDLINE]

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