Curr Opin Biotechnol. 2002 Aug;13(4):297-303.

Recent advances in the application of expressed protein ligation to protein engineering.

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Laboratory of Synthetic Protein Chemistry, The Rockefeller University, New York, NY 10021, USA.

Abstract

Expressed protein ligation is a technique for joining recombinantly expressed proteins to polypeptides containing biophysical probes, post-translational modifications or unnatural amino acids. Recent advances have expanded the scope of expressed protein ligation and have allowed the approach to be applied to the study of basic biological questions.

PMID:: 12323349; [PubMed - indexed for MEDLINE]

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Review Semisynthesis of proteins by expressed protein ligation. [Annu Rev Biochem. 2003]
Review Semisynthesis of proteins by expressed protein ligation.
Muir TW. Annu Rev Biochem. 2003; 72:249-89. Epub 2003 Feb 27.
Review Expressed protein ligation: a resourceful tool to study protein structure and function. [Cell Mol Life Sci. 2009]
Review Expressed protein ligation: a resourceful tool to study protein structure and function.
Berrade L, Camarero JA. Cell Mol Life Sci. 2009 Dec; 66(24):3909-22.
Review Protein semisynthesis and expressed protein ligation: chasing a protein's tail. [Curr Opin Chem Biol. 2005]
Review Protein semisynthesis and expressed protein ligation: chasing a protein's tail.
Schwarzer D, Cole PA. Curr Opin Chem Biol. 2005 Dec; 9(6):561-9. Epub 2005 Oct 13.
Native chemical ligation of polypeptides. [Curr Protoc Protein Sci. 2001]
Native chemical ligation of polypeptides.
Camarero JA, Muir TW. Curr Protoc Protein Sci. 2001 May; Chapter 18:Unit18.4.
Expressed protein ligation: a general method for protein engineering. [Proc Natl Acad Sci U S A. 1998]
Expressed protein ligation: a general method for protein engineering.
Muir TW, Sondhi D, Cole PA. Proc Natl Acad Sci U S A. 1998 Jun 9; 95(12):6705-10.

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Cited by 4 PubMed Central articles

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Reconstitution of modular PDK1 functions on trans-splicing of the regulatory PH and catalytic kinase domains. [Bioconjug Chem. 2007]
Reconstitution of modular PDK1 functions on trans-splicing of the regulatory PH and catalytic kinase domains.
Al-Ali H, Ragan TJ, Gao X, Harris TK. Bioconjug Chem. 2007 Jul-Aug; 18(4):1294-302. Epub 2007 May 15.
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