J Biol Chem. 2002 Nov 29;277(48):46402-7. Epub 2002 Sep 15.

The active principle of garlic at atomic resolution.

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Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany.

Abstract

Despite the fact that many cultures around the world value and utilize garlic as a fundamental component of their cuisine as well as of their medicine cabinets, relatively little is known about the plant's protein configuration that is responsible for the specific properties of garlic. Here, we report the three-dimensional structure of the garlic enzyme alliinase at 1.5 A resolution. Alliinase constitutes the major protein component in garlic bulbs, and it is able to cleave carbon-sulfur bonds. The active enzyme is a pyridoxal-5'-phosphate-dependent homodimeric glycoprotein and belongs to the class I family of pyridoxal-5'-phosphate-dependent enzymes. In addition, it contains a novel epidermal growth factor-like domain that makes it unique among all pyridoxal-5'-phosphate-dependent enzymes.

PMID:: 12235163; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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PDB/1LK9

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Purification, characterization, and crystallization of alliinase from garlic. [Arch Biochem Biophys. 2002]
Purification, characterization, and crystallization of alliinase from garlic.
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Cited by 7 PubMed Central articles

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Alliinase from Ensifer adhaerens and Its Use for Generation of Fungicidal Activity.
Yutani M, Taniguchi H, Borjihan H, Ogita A, Fujita K, Tanaka T. AMB Express. 2011 Mar 28; 1(1):2. Epub 2011 Mar 28.
vanishing tassel2 encodes a grass-specific tryptophan aminotransferase required for vegetative and reproductive development in maize. [Plant Cell. 2011]
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Phillips KA, Skirpan AL, Liu X, Christensen A, Slewinski TL, Hudson C, Barazesh S, Cohen JD, Malcomber S, McSteen P. Plant Cell. 2011 Feb; 23(2):550-66. Epub 2011 Feb 18.
The TRANSPORT INHIBITOR RESPONSE2 gene is required for auxin synthesis and diverse aspects of plant development. [Plant Physiol. 2009]
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Structures reported by this article

The Three-Dimensional Structure Of Alliinase From Garlic

PDB: 1LK9

Source: Allium sativum

Method: X-Ray Diffraction

Resolution: 1.53 Å

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