Cell Death Differ. 2002 Oct;9(10):1140-6.

The C-terminal activation domain of the STAT-1 transcription factor is necessary and sufficient for stress-induced apoptosis.

Source

Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.

Abstract

It has previously been demonstrated that the STAT-1 transcription factor plays a key role in apoptosis induced by the cellular regulatory factors interferon gamma and TNF-alpha. Here we demonstrate that cells lacking STAT-1 show reduced cell death/apoptosis in response to stressful stimuli such as heat or ischaemia. Expression of STAT-1 in these cells does not enhance basal cell death but restores sensitivity to stress-induced death whereas this effect is not observed upon over-expression of STAT-3. Enhanced sensitivity to stress-induced cell death requires the C-terminal activation domain of STAT-1 and the phosphorylation sites at tyrosine 701 and serine 727. Moreover, we show for the first time in any system that the isolated C-terminal domain of STAT-1 is able to enhance stress-induced cell death in the absence of the DNA binding domain or any other region of STAT-1. Hence, STAT-1 plays a key role in stress-induced cell death, potentially acting via a novel co-activator-type mechanism and represents a possible therapeutic target for strategies aimed at minimising cell death, for example, following ischaemic injury.

PMID:: 12232802; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Review Role of STAT-1 and STAT-3 in ischaemia/reperfusion injury. [J Cell Mol Med. 2004]
Review Role of STAT-1 and STAT-3 in ischaemia/reperfusion injury.
Stephanou A. J Cell Mol Med. 2004 Oct-Dec; 8(4):519-25.
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90beta gene promoters. [J Biol Chem. 1999]
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90beta gene promoters.
Stephanou A, Isenberg DA, Nakajima K, Latchman DS. J Biol Chem. 1999 Jan 15; 274(3):1723-8.
Suppression of thermotolerance in mumps virus-infected cells is caused by lack of HSP27 induction contributed by STAT-1. [J Biol Chem. 2003]
Suppression of thermotolerance in mumps virus-infected cells is caused by lack of HSP27 induction contributed by STAT-1.
Yokota S, Yokosawa N, Kubota T, Okabayashi T, Arata S, Fujii N. J Biol Chem. 2003 Oct 24; 278(43):41654-60. Epub 2003 Aug 13.
SHP-2 is a dual-specificity phosphatase involved in Stat1 dephosphorylation at both tyrosine and serine residues in nuclei. [J Biol Chem. 2002]
SHP-2 is a dual-specificity phosphatase involved in Stat1 dephosphorylation at both tyrosine and serine residues in nuclei.
Wu TR, Hong YK, Wang XD, Ling MY, Dragoi AM, Chung AS, Campbell AG, Han ZY, Feng GS, Chin YE. J Biol Chem. 2002 Dec 6; 277(49):47572-80. Epub 2002 Sep 22.
Review Activated STAT-1 pathway in the myocardium as a novel therapeutic target in ischaemia/reperfusion injury. [Eur Cytokine Netw. 2002]
Review Activated STAT-1 pathway in the myocardium as a novel therapeutic target in ischaemia/reperfusion injury.
Stephanou A. Eur Cytokine Netw. 2002 Oct-Dec; 13(4):401-3.

See reviews... See all...

Cited by 5 PubMed Central articles

Characterization of human septic sera induced gene expression modulation in human myocytes. [Int J Clin Exp Med. 2009]
Characterization of human septic sera induced gene expression modulation in human myocytes.
Hussein S, Michael P, Brabant D, Omri A, Narain R, Passi K, Ramana CV, Parrillo JE, Kumar A, Parissenti A, et al. Int J Clin Exp Med. 2009 Jun 5; 2(2):131-48. Epub 2009 Jun 5.
Essential role of STAT1 in caspase-independent cell death of activated macrophages through the p38 mitogen-activated protein kinase/STAT1/reactive oxygen species pathway. [Mol Cell Biol. 2005]
Essential role of STAT1 in caspase-independent cell death of activated macrophages through the p38 mitogen-activated protein kinase/STAT1/reactive oxygen species pathway.
Kim HS, Lee MS. Mol Cell Biol. 2005 Aug; 25(15):6821-33.
Soluble receptor (DcR3) and cellular inhibitor of apoptosis-2 (cIAP-2) protect human cytotrophoblast cells against LIGHT-mediated apoptosis. [Am J Pathol. 2004]
Soluble receptor (DcR3) and cellular inhibitor of apoptosis-2 (cIAP-2) protect human cytotrophoblast cells against LIGHT-mediated apoptosis.
Gill RM, Hunt JS. Am J Pathol. 2004 Jul; 165(1):309-17.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The C-terminal activation domain of the STAT-1 transcription factor is necessary...
The C-terminal activation domain of the STAT-1 transcription factor is necessary and sufficient for stress-induced apoptosis.
Cell Death Differ. 2002 Oct ;9(10):1140-6.

PubMed
Isolation of AmphiCASP-3/7, an ancestral caspase from amphioxus (Branchiostoma f...
Isolation of AmphiCASP-3/7, an ancestral caspase from amphioxus (Branchiostoma floridae). Evolutionary considerations for vertebrate caspases.
Cell Death Differ. 2002 Oct ;9(10):1078-89.

PubMed
Ventilatory function in normal Nigerian school children.
Ventilatory function in normal Nigerian school children.
Afr J Med Med Sci. 1979 Mar-Jun ;8(1-2):25-9.

PubMed
Association of a common allelic polymorphism (C677T) in the methylene tetrahydro...
Association of a common allelic polymorphism (C677T) in the methylene tetrahydrofolate reductase gene with a reduced risk of osteoporotic fractures. A case control study in Danish postmenopausal women.
Calcif Tissue Int. 2002 Nov ;71(5):386-92. Epub 2002 Sep 18 .

PubMed
Calcifying fibrous 'pseudotumor': clinicopathologic study of 15 cases and analys...
Calcifying fibrous 'pseudotumor': clinicopathologic study of 15 cases and analysis of its relationship to inflammatory myofibroblastic tumor.
Int J Surg Pathol. 2002 Jul ;10(3):189-96.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: