FEBS Lett. 2002 Sep 11;527(1-3):143-6.

Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180.

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Abteilung Biochemie, Institut für Molekulare Biotechnologie, Abteilung Biochemie, Beutenbergstr. 11, D-07745, Jena, Germany

Abstract

DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the interaction with the primase subunits, p48 and p58. Furthermore, we demonstrate that exchanging these residues to those of mouse origin can only partially rescue the SV40 DNA replication activity of DNA polymerase alpha-primase.

PMID:: 12220650; [PubMed - indexed for MEDLINE]

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Species-specific replication of simian virus 40 DNA in vitro requires the p180 subunit of human DNA polymerase alpha-primase. [Mol Cell Biol. 1996]
Species-specific replication of simian virus 40 DNA in vitro requires the p180 subunit of human DNA polymerase alpha-primase.
Stadlbauer F, Voitenleitner C, Brückner A, Fanning E, Nasheuer HP. Mol Cell Biol. 1996 Jan; 16(1):94-104.
Protein-protein interactions of the primase subunits p58 and p48 with simian virus 40 T antigen are required for efficient primer synthesis in a cell-free system. [J Biol Chem. 2000]
Protein-protein interactions of the primase subunits p58 and p48 with simian virus 40 T antigen are required for efficient primer synthesis in a cell-free system.
Weisshart K, Förster H, Kremmer E, Schlott B, Grosse F, Nasheuer HP. J Biol Chem. 2000 Jun 9; 275(23):17328-37.
Molecular architecture of the mouse DNA polymerase alpha-primase complex. [Mol Cell Biol. 1999]
Molecular architecture of the mouse DNA polymerase alpha-primase complex.
Mizuno T, Yamagishi K, Miyazawa H, Hanaoka F. Mol Cell Biol. 1999 Nov; 19(11):7886-96.
Review The DNA polymerase alpha-primase complex: multiple functions and interactions. [ScientificWorldJournal. 2003]
Review The DNA polymerase alpha-primase complex: multiple functions and interactions.
Muzi-Falconi M, Giannattasio M, Foiani M, Plevani P. ScientificWorldJournal. 2003 Mar 17; 3:21-33. Epub 2003 Mar 17.
Review The DNA polymerase alpha-primase complex couples DNA replication, cell-cycle progression and DNA-damage response. [Trends Biochem Sci. 1997]
Review The DNA polymerase alpha-primase complex couples DNA replication, cell-cycle progression and DNA-damage response.
Foiani M, Lucchini G, Plevani P. Trends Biochem Sci. 1997 Nov; 22(11):424-7.

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Cited by 4 PubMed Central articles

A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome. [J Biol Chem. 2012]
A conserved motif in the C-terminal tail of DNA polymerase α tethers primase to the eukaryotic replisome.
Kilkenny ML, De Piccoli G, Perera RL, Labib K, Pellegrini L. J Biol Chem. 2012 Jul 6; 287(28):23740-7. Epub 2012 May 16.
Flexible tethering of primase and DNA Pol α in the eukaryotic primosome. [Nucleic Acids Res. 2011]
Flexible tethering of primase and DNA Pol α in the eukaryotic primosome.
Núñez-Ramírez R, Klinge S, Sauguet L, Melero R, Recuero-Checa MA, Kilkenny M, Perera RL, García-Alvarez B, Hall RJ, Nogales E, et al. Nucleic Acids Res. 2011 Oct; 39(18):8187-99. Epub 2011 Jun 28.
Characterization of the 3' exonuclease subunit DP1 of Methanococcus jannaschii replicative DNA polymerase D. [Nucleic Acids Res. 2004]
Characterization of the 3' exonuclease subunit DP1 of Methanococcus jannaschii replicative DNA polymerase D.
Jokela M, Eskelinen A, Pospiech H, Rouvinen J, Syväoja JE. Nucleic Acids Res. 2004; 32(8):2430-40. Epub 2004 Apr 30.

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