Abstract
The 1,670-bp nucleotide sequence of the heat shock operon groESL and the 1,236-bp sequence of the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys were determined. The topology of the groEL- and gltA-based phylogenetic tree was similar to that derived from 16S rRNA gene analyses with distances. Both groESL- and gltA-based PCRs specific to A. platys were also developed based upon the alignment data.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anaplasma / genetics*
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Animals
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Bacterial Proteins / genetics*
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Chaperonins / genetics*
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Citrate (si)-Synthase / genetics*
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Dogs
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Ehrlichiosis / diagnosis*
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Molecular Sequence Data
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Phylogeny*
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / genetics
Substances
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Bacterial Proteins
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GroESL protein, Bacteria
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RNA, Ribosomal, 16S
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Citrate (si)-Synthase
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Chaperonins
Associated data
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GENBANK/AB058782
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GENBANK/AY044161