Invest New Drugs. 2002 Aug;20(3):253-9.

A novel high-through-put assay for screening of pro-apoptotic drugs.

Hägg M, Bivén K, Ueno T, Rydlander L, Björklund P, Wiman KG, Shoshan M, Linder S.

Source

Cancer Center Karolinska, Department of Oncology and Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.

Abstract

Screening for anti-cancer substances is commonly conducted using viability assays. An inherent problem with this approach is that all compounds that are toxic and growth inhibitory, irrespective of mechanism of action, will score positive. It would be beneficial to be able to screen for compounds that specifically induce apoptosis. We here describe an ELISA-assay based on a monoclonal antibody (M30) which recognizes a neo-epitope on cytokeratin 18 exposed after cleavage by caspases during apoptosis. We show that this assay detects apoptosis in epithelial cells and that the sensitivity is sufficient for screening in the 96-well format. We used the M30-ELISA assay to screen 500 low molecular weight compounds from a chemical library from the National Cancer Institute and identified 16 drugs with strong pro-apoptotic activity, suggesting that the assay is a useful tool for discovery of pro-apoptotic drugs.

PMID:: 12201488; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

IndexCopernicus

Supplemental Content

Save items

Related citations in PubMed

A novel assay for discovery and characterization of pro-apoptotic drugs and for monitoring apoptosis in patient sera. [Apoptosis. 2003]
A novel assay for discovery and characterization of pro-apoptotic drugs and for monitoring apoptosis in patient sera.
Bivén K, Erdal H, Hägg M, Ueno T, Zhou R, Lynch M, Rowley B, Wood J, Zhang C, Toi M, et al. Apoptosis. 2003 Jun; 8(3):263-8.
Enzyme-linked immunosorbent assay (ELISA) for the specific detection of apoptotic cells and its application to rapid drug screening. [J Immunol Methods. 2001]
Enzyme-linked immunosorbent assay (ELISA) for the specific detection of apoptotic cells and its application to rapid drug screening.
Frankfurt OS, Krishan A. J Immunol Methods. 2001 Jul 1; 253(1-2):133-44.
Apoptosis enzyme-linked immunosorbent assay distinguishes anticancer drugs from toxic chemicals and predicts drug synergism. [Chem Biol Interact. 2003]
Apoptosis enzyme-linked immunosorbent assay distinguishes anticancer drugs from toxic chemicals and predicts drug synergism.
Frankfurt OS, Krishan A. Chem Biol Interact. 2003 Mar 6; 145(1):89-99.
Review Discovery of 4-aryl-4H-chromenes as potent apoptosis inducers using a cell- and caspase-based Anti-cancer Screening Apoptosis Program (ASAP): SAR studies and the identification of novel vascular disrupting agents. [Anticancer Agents Med Chem. 2009]
Review Discovery of 4-aryl-4H-chromenes as potent apoptosis inducers using a cell- and caspase-based Anti-cancer Screening Apoptosis Program (ASAP): SAR studies and the identification of novel vascular disrupting agents.
Cai SX, Drewe J, Kemnitzer W. Anticancer Agents Med Chem. 2009 May; 9(4):437-56.
Review Screening for apoptosis--classical and emerging techniques. [Anticancer Drugs. 2005]
Review Screening for apoptosis--classical and emerging techniques.
Lövborg H, Gullbo J, Larsson R. Anticancer Drugs. 2005 Jul; 16(6):593-9.

See reviews... See all...

Cited by 14 PubMed Central articles

Interleukin-6 as a therapeutic target in human ovarian cancer. [Clin Cancer Res. 2011]
Interleukin-6 as a therapeutic target in human ovarian cancer.
Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, et al. Clin Cancer Res. 2011 Sep 15; 17(18):6083-96. Epub 2011 Jul 27.
Interactions between IGF-I, estrogen receptor-α (ERα), and ERβ in regulating growth/apoptosis of MCF-7 human breast cancer cells. [J Endocrinol. 2011]
Interactions between IGF-I, estrogen receptor-α (ERα), and ERβ in regulating growth/apoptosis of MCF-7 human breast cancer cells.
Mendoza RA, Enriquez MI, Mejia SM, Moody EE, Thordarson G. J Endocrinol. 2011 Jan; 208(1):1-9. Epub 2010 Oct 25.
Tumorigenicity of MCF-7 human breast cancer cells lacking the p38α mitogen-activated protein kinase. [J Endocrinol. 2011]
Tumorigenicity of MCF-7 human breast cancer cells lacking the p38α mitogen-activated protein kinase.
Mendoza RA, Moody EE, Enriquez MI, Mejia SM, Thordarson G. J Endocrinol. 2011 Jan; 208(1):11-9. Epub 2010 Oct 25.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A novel high-through-put assay for screening of pro-apoptotic drugs.
A novel high-through-put assay for screening of pro-apoptotic drugs.
Invest New Drugs. 2002 Aug ;20(3):253-9.

PubMed
Effect of radiographic techniques (kVp and mAs) on image quality and patient dos...
Effect of radiographic techniques (kVp and mAs) on image quality and patient doses in digital subtraction angiography.
Med Phys. 2002 Aug ;29(8):1643-50.

PubMed
Estimates of radiological risk from a terrorist attack using plutonium.
Estimates of radiological risk from a terrorist attack using plutonium.
Radiat Environ Biophys. 2002 Jun ;41(2):125-30.

PubMed
Efficacy of a 1-week course of proton-pump inhibitor-based triple therapy for er...
Efficacy of a 1-week course of proton-pump inhibitor-based triple therapy for eradicating Helicobacter pylori in patients with and without chronic renal failure.
Am J Kidney Dis. 2002 Sep ;40(3):576-81.

PubMed
beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variab...
beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels.
Thorax. 2002 Sep ;57(9):765-70.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

A novel high-through-put assay for screening of pro-apoptotic drugs.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 14 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information