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Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12173-8. Epub 2002 Aug 26.

Cytochrome c folding pathway: kinetic native-state hydrogen exchange.

Author information

  • 1The Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA. lhoang@mail.med.upenn.edu

Abstract

Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each. Results obtained define a cytochrome c intermediate seen only poorly before and place it early on the major unfolding pathway. Four distinct unfolding steps are found to be kinetically ordered in the same pathway sequence inferred before.

PMID:
12196629
[PubMed - indexed for MEDLINE]
PMCID:
PMC129417
Free PMC Article
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