Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cell. 2002 Aug 9;110(3):315-25.

Genomic deletions created upon LINE-1 retrotransposition.

Author information

  • 1Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA. gilbertn@umich.edu

Abstract

LINE-1 (L1) retrotransposition continues to impact the human genome, yet little is known about how L1 integrates into DNA. Here, we developed a plasmid-based rescue system and have used it to recover 37 new L1 retrotransposition events from cultured human cells. Sequencing of the insertions revealed the usual L1 structural hallmarks; however, in four instances, retrotransposition generated large target site deletions. Remarkably, three of those resulted in the formation of chimeric L1s, containing the 5' end of an endogenous L1 fused precisely to our engineered L1. Thus, our data demonstrate multiple pathways for L1 integration in cultured cells, and show that L1 is not simply an insertional mutagen, but that its retrotransposition can result in significant deletions of genomic sequence.

Comment in

PMID:
12176319
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk