Warning: The NCBI web site requires JavaScript to function. more...
Generate a file for use with external citation management software.
Howard Hughes Medical Institute, Health Research, Incorporated, Albany, NY 12201-0509, USA. joachim@wadsworth.org
Over the past 5 years, thanks to advances in both instrumentation and computational speed, three-dimensional imaging techniques using the electron microscope have been greatly improved in two areas: electron tomography of cell organelles or cell sections and reconstruction of macromolecules from single particles. Ice embedment has brought a breakthrough in the degree of preservation of specimens under close-to-native conditions. The current challenge is to push the resolution of electron tomographic imaging to a point where macromolecular signatures can be recognized within the cellular context. We show first progress toward this goal by examples in two areas of application: the structure of the muscle triad junction and the architecture and fine structure of mitochondria. As techniques of cryo-microtomy are perfected, we hope to be able to apply tomography to high-pressure frozen sections of tissue.
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on