Format

Send to:

Choose Destination
See comment in PubMed Commons below
Calcif Tissue Int. 2002 Sep;71(3):243-8. Epub 2002 Aug 6.

Hydrogen peroxide induces apoptosis of osteocytes: involvement of calcium ion and caspase activity.

Author information

  • 1Department of Orthopaedic Surgery, Kinki University School of Medicine, Ohno-higashi 377-2, Osaka-sayama, Osaka 589-8511, Japan.

Abstract

We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activation of caspase-3 and increase in cytosolic Ca2+. Treatment with BAPTA/AM (cheletor of cytosolic Ca2+) and Ac-DEVD-cho (caspase inhibitor) attenuated hydrogen peroxide-induced apoptosis. These data demonstrated the signal transduction pathways that participate in this hydrogen peroxide-induced cell damage.

PMID:
12154390
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk