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Calcif Tissue Int. 2002 Sep;71(3):243-8. Epub 2002 Aug 6.

Hydrogen peroxide induces apoptosis of osteocytes: involvement of calcium ion and caspase activity.

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  • 1Department of Orthopaedic Surgery, Kinki University School of Medicine, Ohno-higashi 377-2, Osaka-sayama, Osaka 589-8511, Japan.


We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activation of caspase-3 and increase in cytosolic Ca2+. Treatment with BAPTA/AM (cheletor of cytosolic Ca2+) and Ac-DEVD-cho (caspase inhibitor) attenuated hydrogen peroxide-induced apoptosis. These data demonstrated the signal transduction pathways that participate in this hydrogen peroxide-induced cell damage.

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