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Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10611-6. Epub 2002 Jul 29.

Global analysis of stress-regulated mRNA turnover by using cDNA arrays.

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  • 1Laboratory of Cellular and Molecular Biology and DNA Array Unit, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD 21224-6825, USA.


cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, compared with current individual gene-by-gene analyses. This method, which involves comparison of large-scale hybridization patterns generated with steady-state mRNA versus newly transcribed (nuclear run-on) RNA, was used to demonstrate the importance of mRNA turnover in regulating gene expression following several conditions of stress.

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