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J Am Chem Soc. 2002 Aug 7;124(31):9026-7.

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli.

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  • 1Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Abstract

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.

PMID:
12148987
[PubMed - indexed for MEDLINE]
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