J Agric Food Chem. 2002 Jul 31;50(16):4437-44.

High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format.

Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Prior RL.

Source

Brunswick Laboratories, 6 Thatcher Lane, Wareham, Massachusetts 02571, USA.

Abstract

The oxygen radical absorbance capacity (ORAC) assay has been widely accepted as a standard tool to measure the antioxidant activity in the nutraceutical, pharmaceutical, and food industries. However, the ORAC assay has been criticized for a lack of accessibility due to the unavailability of the COBAS FARA II analyzer, an instrument discontinued by the manufacturer. In addition, the manual sample preparation is time-consuming and labor-intensive. The objective of this study was to develop a high-throughput instrument platform that can fully automate the ORAC assay procedure. The new instrument platform consists of a robotic eight-channel liquid handling system and a microplate fluorescence reader. By using the high-throughput platform, the efficiency of the assay is improved with at least a 10-fold increase in sample throughput over the current procedure. The mean of intra- and interday CVs was <or=15%, and the limit of detection and limit of quantitation were 5 and 6.25 microM, respectively.

PMID:: 12137457; [PubMed - indexed for MEDLINE]

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