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J Cell Biol. 2002 Jul 22;158(2):259-71. Epub 2002 Jul 22.

Transcriptome profiling to identify genes involved in peroxisome assembly and function.

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  • 1The Institute for Systems Biology, 1441 N. 34th Street, Seattle, WA 98103-8904, USA.

Abstract

Yeast cells were induced to proliferate peroxisomes, and microarray transcriptional profiling was used to identify PEX genes encoding peroxins involved in peroxisome assembly and genes involved in peroxisome function. Clustering algorithms identified 224 genes with expression profiles similar to those of genes encoding peroxisomal proteins and genes involved in peroxisome biogenesis. Several previously uncharacterized genes were identified, two of which, YPL112c and YOR084w, encode proteins of the peroxisomal membrane and matrix, respectively. Ypl112p, renamed Pex25p, is a novel peroxin required for the regulation of peroxisome size and maintenance. These studies demonstrate the utility of comparative gene profiling as an alternative to functional assays to identify genes with roles in peroxisome biogenesis.

PMID:
12135984
[PubMed - indexed for MEDLINE]
PMCID:
PMC2173120
Free PMC Article
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